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KMID : 0379120120400040224
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Korean Journal of Mycology
2012 Volume.40 No. 4 p.224 ~ p.228
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Analysis of Protein Domain for Interaction between α-COP and ε-COP in Aspergillus nidulans
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Song Eun-Jung
Kim Ki-Hyun
Lee Hwan-Hee
Park Jeong-Seok
Kang Eun-Hye
Park Hee-Moon
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Abstract
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In order to screen interactor(s) of the Aspergillus nidulans α-COP of COPI vesicle, we performed the yeast two hybrid screening by using the gene for A. nidulans α-COP as a bait and identified ε-COP of the COPI vesicle as an interacting protein. The A. nidualns gene for the ε-COP was designated aneA+ (A. nidulans epsilone-COP), which encoded 296 amino acid residues with high level of identity with orthologs from other fungi. Domain analyses with yeast two-hybrid system suggested that the interaction between α-COP and ε-COP relied on the C-terminus of both proteins, and that the N-terminal WD domian of α-COP and the TPR region of ε-COP were not essential but required for the enhancement of the interaction. These results indicate that the interaction mode between α-COP and ε-COP of COPI vesicle is evolutionarily well conserved in eukaryotes.
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KEYWORD
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Aspergillus nidulans, α-COP, ε-COP, Yeast two-hybrid assay
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